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- LB Broth, Sterile, 100 mL
LB Broth, Sterile, 100 mL
DKK 367
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Ultrapure-Sterile LB broth out-performs autoclaved media for all your bacteria culture needs!
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Produktdetaljer
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 7.52 ounces (213.19 grams) |
| Volume | 100 ml |
| Brand Name | ABI |
| Manufacturer | American BioInnovations |
| Part Number | ABI-106-03 |
| Brand | ABI |
| Item Volume | 100 ml |
| Package Information | Bottle |
Hvem bør købe?
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Microbiology Labs
Ideal for sterile culture media in microbiological experiments, supporting bacterial growth for research and testing purposes.
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Educational Institutions
Useful in universities for teaching microbiology, providing students with hands-on experience in culturing bacteria safely.
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Clinical Research
Beneficial for researchers working on clinical studies requiring controlled bacterial cultures without contamination risks.
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Home Use
Not suitable for casual home experiments where sterile conditions and proper lab equipment cannot be guaranteed.
Produktbeskrivelse
LB Broth, Sterile, 100 mL
Kundespørgsmål og svar
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spørgsmål:
What is LB Broth and what is it used for?
svar: LB Broth, or Luria-Bertani Broth, is a nutrient-rich medium widely used for the growth of bacteria in laboratories. It provides the necessary nutrients, including peptides and salts, that support robust bacterial growth, making it essential in microbiology for experiments involving cloning, plasmid propagation, and protein expression. Labs often use LB Broth for cultivating E. coli or other microorganisms for research in genetic studies and biotechnology applications. -
spørgsmål:
Is LB Broth, Sterile, 100 ML suitable for sensitive experiments?
svar: Yes, LB Broth, Sterile, 100 ML is specifically designed for sensitive experiments. Its sterile formulation eliminates the risk of contamination, ensuring that your bacterial cultures grow in a controlled environment. This is critical when working with genetically modified organisms or when precise results are required. Utilizing this sterile broth helps maintain the integrity of your experiments and minimizes data variability caused by contaminants. -
spørgsmål:
How should I store LB Broth after opening it?
svar: After opening, LB Broth should be stored at 2–8°C to maintain its stable properties and prevent microbial contamination. It is important to keep it tightly sealed and protected from light to preserve its efficacy. Storing LB Broth correctly ensures that it remains usable for subsequent experiments, especially in cases requiring a specialized medium for bacterial growth. Use it within a reasonable timeframe to enjoy optimal results. -
spørgsmål:
Can I use LB Broth for growing non-E. coli strains?
svar: Yes, LB Broth can be used to grow a variety of bacterial strains, including non-E. coli species. While it is most commonly associated with E. coli, many gram-negative and some gram-positive bacteria can thrive in LB Broth due to its comprehensive nutrient profile. This versatility makes it an ideal choice in laboratories for diverse microbial studies, allowing researchers to explore a wide range of bacteria and their behaviors. -
spørgsmål:
What should I do if the LB Broth appears cloudy or discolored?
svar: If the LB Broth appears cloudy or discolored, it is advisable to discard it as these changes are indicative of contamination or degradation. A clear appearance is essential for reliable experimentation and suggests that the broth is still uncontaminated and usable. Always check for proper color and clarity before use, ensuring that your results remain credible and reproducible in your experiments. -
spørgsmål:
Does LB Broth contain any antibiotics?
svar: LB Broth, in its basic form, does not contain any antibiotics; it is solely a nutrient medium designed for bacterial growth. However, researchers often add antibiotics to LB Broth according to their experimental requirements to select for or against certain bacterial strains. This flexibility allows you to customize the medium based on specific needs, such as studying antibiotic resistance or ensuring the survival of plasmid-bearing bacteria. -
spørgsmål:
How is LB Broth typically prepared?
svar: LB Broth is typically prepared by dissolving specific quantities of its dry ingredients—tryptone, yeast extract, and sodium chloride—in distilled water, followed by adjusting the pH. The mixture is then autoclaved to sterilize it, ensuring it is free from contaminants. This preparation method allows researchers to produce LB Broth tailored to their experimental protocols, offering versatility in microbiological applications. -
spørgsmål:
How long can I store LB Broth once prepared?
svar: Once prepared, LB Broth can typically be stored at 2–8°C for about one week. It is important to monitor for any changes in appearance, as signs of contamination or sediment may indicate it is no longer viable. Properly storing the broth in a sterile container helps ensure that its quality remains high for any future experiments, allowing convenient access and reducing waste of resources in the lab. -
spørgsmål:
What is the typical application of LB Broth in the laboratory?
svar: LB Broth is primarily used for culturing microorganisms, particularly in molecular biology and microbiology. It serves as a medium for various applications, including plasmid purification, cloning experiments, and protein expression in recombinant DNA technology. The nutrient-rich composition supports optimal growth rates, making it ideal for producing large quantities of bacteria needed for experimental procedures. -
spørgsmål:
Where can I buy LB Broth, Sterile, 100 ML?
svar: You can buy LB Broth, Sterile, 100 ML on Ubuy. Ubuy offers a reliable platform for purchasing high-quality laboratory supplies, ensuring you get the products you need for your research with the convenience of online shopping.
ABI Natural Proteins Editorial Review
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DKK 367
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Egenskaber og fordele
- Ideal for liquid bacteria culture and coliphage plaque assays.
- Perfect for recombinant DNA work including protein expression and transformation.
- Filter-sterilized for maximum purity and performance.
- Tested for effective microbiological growth.
- Supports cloning and plasmid expansion.
- A must-have for advanced laboratory applications.
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